Molecular architecture of tight junctions of periderm differs from that of the maculae occludentes of epidermis

J Invest Dermatol. 2002 Jun;118(6):1073-9. doi: 10.1046/j.1523-1747.2002.01774.x.

Abstract

Occludin and claudins are tetraspan-transmembrane proteins in tight junctions. Maculae occludentes, which are less-developed tight junctions, occur in the granular cell layer of the epidermis. The periderm, which overlies the developing epidermis and functions as a protective layer for the embryo, carries developed tight junctions as observed in simple epithelia. In both periderm and epidermis, occludin is expressed at the cell-cell border. To determine the difference between tight junctions of periderm and epidermis, claudin-6 expression was examined in periderm and epidermis. Immunofluorescence staining showed claudin-6 expression at the cell-cell border of the periderm, but not in the epidermis. Reverse transcription-polymerase chain reaction confirmed that claudin-6 was not expressed in mouse adult skin, whereas immunoelectron microscopy revealed that claudin-6 was localized at tight junctions of the periderm. Furthermore, L fibroblasts with stable expression of exogenous claudin-6 formed developed tight junctions at cell-cell borders. These findings indicate that molecular architecture of tight junctions of the periderm is different from that of the maculae occludentes of the epidermis, and that claudin-6 is important in the formation of tight junctions of the periderm.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Claudins
  • Dermis / chemistry
  • Dermis / cytology*
  • Epidermal Cells*
  • Epidermis / chemistry
  • Female
  • Fibroblasts / physiology
  • Freeze Fracturing
  • Male
  • Membrane Proteins / analysis*
  • Membrane Proteins / genetics
  • Mice
  • Microscopy, Immunoelectron
  • Phosphoproteins / analysis
  • Pregnancy
  • Tight Junctions / chemistry*
  • Tight Junctions / ultrastructure
  • Transfection
  • Zonula Occludens-1 Protein
  • Zonula Occludens-2 Protein

Substances

  • Claudins
  • Membrane Proteins
  • Phosphoproteins
  • Tjp1 protein, mouse
  • Tjp2 protein, mouse
  • Zonula Occludens-1 Protein
  • Zonula Occludens-2 Protein
  • claudin 6