An electrochemical detection scheme for identification of single nucleotide polymorphisms using hairpin-forming probes

Nucleic Acids Res. 2002 Jun 15;30(12):e55. doi: 10.1093/nar/gnf054.

Abstract

Single nucleotide polymorphisms are implicated as having a significant role in regulating growth, development and, thereby, human health and disease. We have developed a method for identifying single nucleotide genetic alterations by combining hairpin-forming DNA probes and electrochemical detection of sandwich DNA hybridization. Incorporation of hairpin-forming competitor probes and the catalyzed reporter deposition amplification system further improves assay specificity by 7-fold and sensitivity by 100-fold. We have demonstrated that the system successfully identified the factor V Leiden mutations from human blood specimens.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Probes / chemistry*
  • Electrochemistry / methods*
  • Factor V / genetics
  • Humans
  • Immunoenzyme Techniques / methods
  • Nucleic Acid Conformation
  • Oligonucleotide Probes / chemistry
  • Oligonucleotides / analysis
  • Point Mutation
  • Polymorphism, Single Nucleotide*
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods*

Substances

  • DNA Probes
  • Oligonucleotide Probes
  • Oligonucleotides
  • factor V Leiden
  • Factor V