Regulation of human eotaxin-3/CCL26 expression: modulation by cytokines and glucocorticoids

Cytokine. 2002 Mar 21;17(6):317-23. doi: 10.1006/cyto.2002.1021.


Eotaxin-3 (CCL26) is a CC chemokine that signals exclusively via the CCR3 receptor and has eosinophil-selective chemoattractant activity. Comparison of Eotaxin-1 (CCL11) and Eotaxin-2 (CCL24), demonstrates differences in their expression profiles, cell specificity and effector kinetics, implying distinct biological actions. But little data in this regard have been reported for Eotaxin-3. We aimed to analyse the effect of Th2 cytokines and glucocorticoids on Eotaxin-3 mRNA expression in human lung epithelial cells and dermal fibroblasts; cells implicated in the pathogenesis of allergic asthma and allergic dermatitis respectively. Eotaxin-3 mRNA levels in primary dermal fibroblasts and NCI-H727 lung epithelial cells were determined by Northern hybridization. In contrast to Eotaxin-1, Eotaxin-3 mRNA expression was not detected in unstimulated cells. The Th2 cytokines IL-4 and IL-13 induced Eotaxin-3 expression in a time and dose dependent manner, with IL-4 demonstrating a 100-fold greater potency. Unlike Eotaxin-1, Eotaxin-3 mRNA expression was not induced by either tumour necrosis factor (TNF)-alpha or interleukin (IL)-1 beta alone. Both IL-4 and IL-13 acted synergistically with TNF-alpha in superinducing Eotaxin-3 mRNA expression. Dexamethasone pre-treatment diminished induction of Eotaxin-3 mRNA expression. We conclude that modulation of Eotaxin-3 mRNA expression by Th(2) cytokines is different from that of Eotaxin-1 and Eotaxin-2, further supporting a distinct biological role for Eotaxin-3.

MeSH terms

  • Cell Line
  • Chemokine CCL11
  • Chemokine CCL24
  • Chemokine CCL26
  • Chemokines, CC / genetics*
  • Cytokines / pharmacology*
  • Dexamethasone / pharmacology*
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology*
  • Glucocorticoids / pharmacology*
  • Humans
  • Interleukin-13 / pharmacology
  • Interleukin-4 / pharmacology
  • Kinetics
  • Lung / physiology*
  • Respiratory Mucosa / physiology*
  • Transcription, Genetic / drug effects


  • CCL11 protein, human
  • CCL24 protein, human
  • CCL26 protein, human
  • Chemokine CCL11
  • Chemokine CCL24
  • Chemokine CCL26
  • Chemokines, CC
  • Cytokines
  • Glucocorticoids
  • Interleukin-13
  • Interleukin-4
  • Dexamethasone