Latent TGF-beta binding protein-3 (LTBP-3) requires binding to TGF-beta for secretion

FEBS Lett. 2002 Apr 24;517(1-3):277-80. doi: 10.1016/s0014-5793(02)02648-0.

Abstract

Latent transforming growth factor-beta (TGF-beta) binding protein (LTBP)-1, which is easily secreted, has been shown to enhance the secretion of TGF-beta. Here we show that another member of the LTBP family, LTBP-3, is not secreted by several cell types, but secretion occurs after coexpression with TGF-beta. The secretion of LTBP-3 requires complexing of LTBP-3 with Cys33 of the TGF-beta propeptide.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cells, Cultured
  • Cysteine / metabolism*
  • Disulfides / metabolism*
  • Humans
  • Intracellular Signaling Peptides and Proteins*
  • Latent TGF-beta Binding Proteins
  • Mice
  • Protein Binding
  • Recombinant Fusion Proteins / metabolism
  • Transfection
  • Transforming Growth Factor beta / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Disulfides
  • Intracellular Signaling Peptides and Proteins
  • LTBP1 protein, human
  • LTBP3 protein, human
  • Latent TGF-beta Binding Proteins
  • Ltbp1 protein, mouse
  • Ltbp3 protein, mouse
  • Recombinant Fusion Proteins
  • Transforming Growth Factor beta
  • Cysteine