Towards quantitative assays with peptide chips: a surface engineering approach

Trends Biotechnol. 2002 Jul;20(7):279-81. doi: 10.1016/s0167-7799(02)01984-4.

Abstract

The development of peptide and protein microarrays has created enormous opportunities in biomedical research. Current chip-based assays are well suited for identifying candidate protein or enzyme activities but still require conventional solution phase experiments to validate hits. Here, three surface-engineering strategies for microarray design are described and are illustrated in the development of a peptide chip for the quantitative analysis of kinase activity on solid support. These strategies promise to widen the application of microarrays by permitting the evaluation of hits in a chip-based format.

MeSH terms

  • Amino Acid Sequence
  • Enzymes, Immobilized / chemistry
  • Molecular Sequence Data
  • Peptides / chemistry*
  • Protein Array Analysis / instrumentation*
  • Protein Array Analysis / methods
  • Protein Array Analysis / trends
  • Protein-Tyrosine Kinases / analysis
  • Protein-Tyrosine Kinases / chemistry*
  • Substrate Specificity
  • Surface Properties

Substances

  • Enzymes, Immobilized
  • Peptides
  • Protein-Tyrosine Kinases