Abstract
We assessed in vivo the anti-inflammatory activity of two Cat's claw bark extracts, by comparing a spray-dried hydroalcoholic extract against an aqueous freeze-dried extract, to determine which extract was more effective. We used the carrageenan-induced paw edema model in mice. In addition, to assess the molecular mechanism of action, we determined the inhibition of NF-kappa B through the Electrophoretic Mobility Shift Assay (EMSA) and the effects on cycloxygenase-1 and -2. Results showed that the anti-inflammatory activity was significantly higher using the hydroalcoholic compared with the aqueous extract (P<0.05). The extracts also showed little inhibitory activity on cyclooxygenase-1 and -2. It cannot be excluded that the slight inhibitory activity on DNA binding of NF-kappa B is due to cytotoxic effects.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Cat's Claw*
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Cyclooxygenase 1
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Cyclooxygenase 2
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Edema / chemically induced
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Edema / drug therapy
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Enzyme Inhibitors / pharmacology
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Female
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Humans
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Isoenzymes / antagonists & inhibitors
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Isoenzymes / metabolism
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Jurkat Cells / drug effects
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Membrane Proteins
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Mice
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Mice, Inbred BALB C
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NF-kappa B / metabolism
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Plant Bark
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Prostaglandin-Endoperoxide Synthases / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Enzyme Inhibitors
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Isoenzymes
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Membrane Proteins
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NF-kappa B
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Plant Extracts
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Cyclooxygenase 1
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Cyclooxygenase 2
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PTGS1 protein, human
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PTGS2 protein, human
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Prostaglandin-Endoperoxide Synthases
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Ptgs1 protein, mouse