Homolog interaction during meiotic prophase I in Arabidopsis requires the SOLO DANCERS gene encoding a novel cyclin-like protein

EMBO J. 2002 Jun 17;21(12):3081-95. doi: 10.1093/emboj/cdf285.

Abstract

Interactions between homologs in meiotic prophase I, such as recombination and synapsis, are critical for proper homolog segregation and involve the coordination of several parallel events. However, few regulatory genes have been identified; in particular, it is not clear what roles the proteins similar to the mitotic cell cycle regulators might play during meiotic prophase I. We describe here the isolation and characterization of a new Arabidopsis mutant called solo dancers that exhibits a severe defect in homolog synapsis, recombination and bivalent formation in meiotic prophase I, subsequently resulting in seemingly random chromosome distribution and formation of abnormal meiotic products. We further demonstrate that the mutation affects a meiosis-specific gene encoding a novel protein of 578 amino acid residues with up to 31% amino acid sequence identity to known cyclins in the C-terminal portion. These results argue strongly that homolog interactions during meiotic prophase I require a novel meiosis-specific cyclin in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / anatomy & histology
  • Arabidopsis / genetics*
  • Arabidopsis / physiology*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Chromosomes / metabolism
  • Cyclins / chemistry
  • Cyclins / genetics
  • Cyclins / metabolism*
  • Genes, Plant*
  • In Situ Hybridization
  • Meiosis / physiology*
  • Molecular Sequence Data
  • Phenotype
  • Plants / anatomy & histology
  • Plants / metabolism
  • Recombination, Genetic
  • Sequence Alignment
  • Two-Hybrid System Techniques

Substances

  • Arabidopsis Proteins
  • Cyclins
  • solo dancers protein, Arabidopsis

Associated data

  • GENBANK/AJ457977