Specific induction of apoptosis by 1,8-cineole in two human leukemia cell lines, but not a in human stomach cancer cell line

Oncol Rep. Jul-Aug 2002;9(4):757-60.


We have investigated the effects of 1,8-cineole [the main component of essential oil prepared from bay-leaves Laurus nobilis L.)] on DNA of human leukemia cell lines, Molt 4B, HL-60 and stomach cancer KATO III cells. Specific induction of apoptosis by 1,8-cineole was observed in human leukemia Molt 4B and HL-60 cells, but not in human stomach cancer KATO III cells. Morphological changes showing apoptotic bodies were observed in the human leukemia HL-60 cells treated with 1,8-cineole. The fragmentations of DNA by cineole to oligonucleosomal-sized fragments that is a characteristic of apoptosis were concentration- and time-dependent in Molt 4B and HL-60 cells, but not in KATO III cells. The present study shows that the suppression growth by 1,8-cineole in the leukemia cell lines results from the induction of apoptosis by this compound.

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects*
  • Cyclohexanols / pharmacology*
  • DNA, Neoplasm / drug effects*
  • Eucalyptol
  • Humans
  • Laurus / chemistry
  • Leukemia / pathology*
  • Monoterpenes*
  • Stomach Neoplasms / pathology*
  • Terpenes / pharmacology*
  • Tumor Cells, Cultured / drug effects*
  • Tumor Cells, Cultured / metabolism
  • Tumor Cells, Cultured / pathology


  • Antineoplastic Agents, Phytogenic
  • Cyclohexanols
  • DNA, Neoplasm
  • Monoterpenes
  • Terpenes
  • Eucalyptol