Efficient transduction of dendritic cells and induction of a T-cell response by third-generation lentivectors

Hum Gene Ther. 2002 Jun 10;13(9):1091-100. doi: 10.1089/104303402753812494.


In order to induce a therapeutic T lymphocyte response, recombinant viral vaccines are designed to target professional antigen-presenting cells (APC) such as dendritic cells (DC). A key requirement for their use in humans is safe and efficient gene delivery. The present study assesses third-generation lentivectors with respect to their ability to transduce human and mouse DC and to induce antigen-specific CD8+ T-cell responses. We demonstrate that third-generation lentivectors transduce DC with a superior efficiency compared to adenovectors. The transfer of DC transduced with a recombinant lentivector encoding an antigenic epitope resulted in a strong specific CD8+ T-cell response in mice. The occurrence of lower proportions of nonspecifically activated CD8+ cells suggests a lower antivector immunity of lentivector compared to adenovector. Thus, lentivectors, in addition to their promise for gene therapy of brain disorders might also be suitable for immunotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / immunology
  • Cells, Cultured
  • Dendritic Cells / metabolism*
  • Epitopes, T-Lymphocyte / genetics
  • Epitopes, T-Lymphocyte / immunology
  • Genetic Vectors / genetics*
  • Genetic Vectors / metabolism
  • HIV-1 / genetics
  • Humans
  • Interferon-gamma / metabolism
  • Lentivirus / genetics*
  • Lentivirus / metabolism
  • Mice
  • Mice, Transgenic
  • Phenotype
  • T-Lymphocytes / immunology*
  • Transduction, Genetic*


  • Epitopes, T-Lymphocyte
  • Interferon-gamma