Bacterial lipopolysaccharide induces mRNA expression of an IkappaB MAIL through toll-like receptor 4

J Vet Med Sci. 2002 May;64(5):419-22. doi: 10.1292/jvms.64.419.


Molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL) is a nuclear IkappaB protein recently identified as a molecule appearing in immunocompetent organs after administration of bacterial lipopolysaccharide (LPS). Participation of Toll-like receptor (TLR) 4, which is a major form of LPS receptors, in the LPS-induced MAIL expression was investigated. When a human myelomonocytic cell line U937 was treated with phorbol 12-myristate 13-acetate for 3 days, the LPS-induced MAIL expression was much potentiated in parallel with an increase in TLR4 expression. The MAIL induction was attenuated when the cells were treated with a neutralizing antibody against TLR4. The in vivo induction of MAIL in the spleen was smaller in mice having a missense mutation of the Tlr4 gene than in normal control mice. These results collectively indicate that TLR4 contributes, at least in part, MAIL induction after LPS stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Drosophila Proteins*
  • Gene Expression Regulation / drug effects*
  • Humans
  • I-kappa B Proteins
  • Lipopolysaccharides / pharmacology*
  • Male
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Mice, Inbred C3H
  • Nuclear Proteins / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface / metabolism*
  • Toll-Like Receptor 4
  • Toll-Like Receptors
  • Tumor Cells, Cultured
  • U937 Cells


  • Adaptor Proteins, Signal Transducing
  • Drosophila Proteins
  • I-kappa B Proteins
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • NFKBIZ protein, human
  • Nfkbiz protein, mouse
  • Nuclear Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • TLR4 protein, human
  • Toll-Like Receptor 4
  • Toll-Like Receptors