RNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block

Virology. 2002 May 10;296(2):321-9. doi: 10.1006/viro.2001.1328.


Cell-to-cell and long-distance transport of some plant viruses requires coordinated action of three movement proteins encoded by triple gene block (TGB). The largest of TGB proteins, TGBp1, is a member of the superfamily I of DNA/RNA helicases and possesses a set of conserved helicase sequence motifs necessary for virus movement. A recombinant His-tagged form of TGBp1 of two hordeiviruses and potato virus X, a potexvirus, produced in Escherichia coli had unwinding activity on a partially duplexed RNA, but not DNA substrate. The helicase activity of these proteins was dependent on Mg2+ and ATP. The isolated C-terminal half of the PSLV TGBp1 retaining all helicase motifs was also able to unwind RNA duplex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Amino Acid Sequence
  • DNA / metabolism
  • Genes, Viral / physiology
  • Molecular Sequence Data
  • Mosaic Viruses / enzymology*
  • Mosaic Viruses / genetics
  • Plant Viral Movement Proteins
  • Plant Viruses / enzymology
  • Plant Viruses / genetics
  • Potexvirus / enzymology
  • Potexvirus / genetics
  • RNA Helicases / genetics
  • RNA Helicases / metabolism*
  • RNA Viruses / enzymology*
  • RNA Viruses / genetics
  • RNA, Double-Stranded / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*


  • Plant Viral Movement Proteins
  • RNA, Double-Stranded
  • Recombinant Fusion Proteins
  • Viral Proteins
  • DNA
  • TGBp1 protein, Poa semilatent virus
  • Adenosine Triphosphatases
  • RNA Helicases