The T cell, which plays an integral role in the coordination of the immune system, has a heterodimer receptor (TCR) that can exist in one of the two forms: alpha/beta or gamma/delta. Cells displaying the gamma/delta TCR comprise less than 5% of T cell populations in humans and mice. In the bovine system, however, gamma/delta populations can reach as high as 60%. Differences in T cell populations make the bovine system an excellent candidate for genomic TCR sequencing and multi-species comparisons. In an effort to characterize the bovine TCR loci, a genomic library was screened for the beta TCR gene. A shotgun sequencing library was constructed and preliminary analysis demonstrates that the organization of the bovine TCR beta constant regions is different from both humans and mice. The bovine beta locus appears to have a third constant region. Overall, the genomic characterization of the bovine TCR genes will provide insight into the evolution of T cell receptor.