TRPV3 is a calcium-permeable temperature-sensitive cation channel

Nature. 2002 Jul 11;418(6894):181-6. doi: 10.1038/nature00882. Epub 2002 Jun 23.


Transient receptor potential (TRP) proteins are cation-selective channels that function in processes as diverse as sensation and vasoregulation. Mammalian TRP channels that are gated by heat and capsaicin (>43 degrees C; TRPV1 (ref. 1)), noxious heat (>52 degrees C; TRPV2 (ref. 2)), and cooling (< 22 degrees C; TRPM8 (refs 3, 4)) have been cloned; however, little is known about the molecular determinants of temperature sensing in the range between approximately 22 degrees C and 40 degrees C. Here we have identified a member of the vanilloid channel family, human TRPV3 (hTRPV3) that is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain. Increasing temperature from 22 degrees C to 40 degrees C in mammalian cells transfected with hTRPV3 elevated intracellular calcium by activating a nonselective cationic conductance. As in published recordings from sensory neurons, the current was steeply dependent on temperature, sensitized with repeated heating, and displayed a marked hysteresis on heating and cooling. On the basis of these properties, we propose that hTRPV3 is thermosensitive in the physiological range of temperatures between TRPM8 and TRPV1.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Calcium / metabolism*
  • Calcium Signaling
  • Cation Transport Proteins*
  • Cations, Divalent / metabolism
  • Cell Membrane Permeability*
  • Cloning, Molecular
  • Cricetinae
  • Electric Conductivity
  • Electrophysiology
  • Evolution, Molecular
  • Gene Expression Profiling
  • Humans
  • Ion Channel Gating*
  • Ion Channels / chemistry
  • Ion Channels / genetics
  • Ion Channels / metabolism*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment
  • TRPV Cation Channels
  • Temperature*


  • Cation Transport Proteins
  • Cations, Divalent
  • Ion Channels
  • RNA, Messenger
  • TRPV Cation Channels
  • TRPV3 protein, human
  • Calcium