A novel, evolutionarily conserved gene family with putative sequence-specific single-stranded DNA-binding activity

Genomics. 2002 Jul;80(1):78-85. doi: 10.1006/geno.2002.6805.


Complete and partial deletions of chromosome 5q are recurrent cytogenetic anomalies associated with aggressive myeloid malignancies. Earlier, we identified an approximately 1.5-Mb region of loss at 5q13.3 between the loci D5S672 and D5S620 in primary leukemic blasts. A leukemic cell line, ML3, is diploid for all of chromosome 5, except for an inversion-coupled translocation within the D5S672-D5S620 interval. Here, we report the development of a bacterial artificial chromosome (BAC) contig to define the breakpoint and the identification of a novel gene SSBP2, the target of disruption in ML3 cells. A preliminary evaluation of SSBP2 as a tumor suppressor gene in primary leukemic blasts and cell lines suggests that the remaining allele does not undergo intragenic mutations. SSBP2 is one of three members of a closely related, evolutionarily conserved, and ubiquitously expressed gene family. SSBP3 is the human ortholog of a chicken gene, CSDP, that encodes a sequence-specific single-stranded DNA-binding protein. SSBP3 localizes to chromosome 1p31.3, and the third member, SSBP4, maps to chromosome 19p13.1. Chromosomal localization and the putative single-stranded DNA-binding activity suggest that all three members of this family are capable of potential tumor suppressor activity by gene dosage or other epigenetic mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromosomes, Artificial, Bacterial
  • Chromosomes, Human, Pair 5*
  • Conserved Sequence
  • DNA-Binding Proteins / genetics*
  • Evolution, Molecular
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Multigene Family*
  • Physical Chromosome Mapping
  • Sequence Alignment
  • Sequence Deletion
  • Translocation, Genetic
  • Tumor Cells, Cultured


  • DNA-Binding Proteins
  • SSBP2 protein, human