Metastatic Lymph Node 51, a Novel Nucleo-Cytoplasmic Protein Overexpressed in Breast Cancer

Oncogene. 2002 Jun 27;21(28):4422-34. doi: 10.1038/sj.onc.1205611.

Abstract

Metastatic Lymph Node 51 (MLN51) cDNA was isolated by differential screening of a human breast cancer metastasis cDNA library. MLN51 cDNA encodes a novel human protein of 703 residues that shares no significant homology to any known protein. However MLN51 is well conserved between vertebrate and invertebrate species suggesting an important biological function. The amino terminal half of the protein contains a coiled-coil domain and two potential nuclear localization signals (NLS). The carboxy terminal half contains one SH2 and four SH3 binding motifs. The coiled-coil domain promotes MLN51 oligomerization in transfected cells. When transiently expressed, the MLN51 protein is mainly found in the cytoplasm with a weak nuclear staining. However, deletion of the carboxy terminal half of the protein allows the targeting of the protein to the nucleus, demonstrating that the NLSs are functional. MLN51 is ubiquitously expressed in normal tissues. Human breast carcinomas show MLN51 overexpression in malignant epithelial cells. The uncommon association of protein-protein interaction domains often found either in nuclear or in cytoplasmic signaling proteins raises a possible nucleo-cytoplasmic function for MLN51.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Cell Nucleus / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 17 / genetics
  • Cloning, Molecular
  • Cytoplasm / metabolism
  • DNA Primers / chemistry
  • Fluorescent Antibody Technique
  • Humans
  • Immunoenzyme Techniques
  • Lymph Nodes / pathology
  • Lymphatic Metastasis
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / metabolism
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Precipitin Tests
  • RNA-Binding Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured

Substances

  • CASC3 protein, human
  • DNA Primers
  • Neoplasm Proteins
  • Nuclear Proteins
  • RNA-Binding Proteins