MIM, a potential metastasis suppressor gene in bladder cancer

Neoplasia. Jul-Aug 2002;4(4):291-4. doi: 10.1038/sj.neo.7900231.

Abstract

Using a modified version of the mRNA differential display technique, five human bladder cancer cell lines from low grade to metastatic were analyzed to identify differences in gene expression. A 316-bp cDNA (C11-300) was isolated that was not expressed in the metastatic cell line TccSuP. Sequence analysis revealed that this gene was identical to KIAA 0429, has a 5.3-kb transcript that mapped to 8q24.1. The protein is predicted to be 356 amino acids in size and has an actin-binding WH2 domain. Northern blot revealed expression in multiple normal tissues, but none in a metastatic breast cancer cell line (SKBR3) or in metastatic prostatic cancer cell lines (LNCaP, PC3). We have named this gene Missing in Metastasis (MIM) and our data suggest that it may be involved in cytoskeletal organization.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Blotting, Northern
  • Breast Neoplasms / pathology
  • Carcinoma, Transitional Cell / genetics*
  • Carcinoma, Transitional Cell / pathology
  • Chromosomes, Human, Pair 8 / genetics
  • Cytoskeleton / ultrastructure
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Female
  • Gene Expression Profiling
  • Genes, Tumor Suppressor*
  • Humans
  • Male
  • Molecular Sequence Data
  • Multigene Family
  • Neoplasm Metastasis
  • Neoplasm Proteins / genetics*
  • Prostatic Neoplasms / pathology
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / isolation & purification
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Subtraction Technique
  • Testis / metabolism
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms / genetics*
  • Urinary Bladder Neoplasms / pathology

Substances

  • DNA, Complementary
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm