Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis

Judith Rohde; Anja Rothkamp; Gerald F Gerlach

doi:10.1128/JCM.40.7.2598-2600.2002

Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis

J Clin Microbiol. 2002 Jul;40(7):2598-600. doi: 10.1128/JCM.40.7.2598-2600.2002.

Authors

Judith Rohde¹, Anja Rothkamp, Gerald F Gerlach

Affiliation

¹ Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Hannover, Germany. Jurohde@gmx.de

Abstract

A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Typing Techniques
Base Sequence
DNA Primers / genetics
DNA, Bacterial / genetics
Genes, Bacterial
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length
Species Specificity
Spirochaetales / classification*
Spirochaetales / genetics*
Spirochaetales / isolation & purification
Swine / microbiology*

Substances

DNA Primers
DNA, Bacterial