Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 296 (5577), 2395-8

Channelrhodopsin-1: A Light-Gated Proton Channel in Green Algae

Affiliations

Channelrhodopsin-1: A Light-Gated Proton Channel in Green Algae

Georg Nagel et al. Science.

Abstract

Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.

Similar articles

See all similar articles

Cited by 263 PubMed Central articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback