Role of heparan sulfate in human parainfluenza virus type 3 infection

Virology. 2002 Jun 20;298(1):73-83. doi: 10.1006/viro.2002.1484.

Abstract

Our current studies have demonstrated that human parainfluenza virus type 3 (HPIV-3) utilizes heparan sulfate (HS) for its efficient cellular entry. HPIV-3 interacted with HS-agarose in vitro and the cellular entry and infection of HPIV-3 were reduced following (a) infection of human epithelial lung A549 cells with HPIV-3 pre-incubated with soluble HS; (b) treatment of A549 cells with heparinase to remove cell surface HS and sodium chlorate (NaClO(3)), a potent inhibitor of proteoglycan sulfation; and (c) infection of HS-deficient mutant CHO cell lines. However, in each instance, complete inhibition of HPIV-3 entry did not occur, suggesting the presence of additional nonproteoglycan cell surface molecule(s) that is required for HPIV-3 entry. Thus the cell surface HS appears to play an important role in efficient cellular entry of HPIV-3.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CHO Cells / virology
  • Cell Membrane / virology
  • Cricetinae
  • Epithelial Cells / virology*
  • Heparin Lyase / pharmacology
  • Heparitin Sulfate / pharmacology
  • Heparitin Sulfate / physiology*
  • Humans
  • Lung
  • Parainfluenza Virus 3, Human / metabolism
  • Parainfluenza Virus 3, Human / physiology*
  • Virus Replication

Substances

  • Heparitin Sulfate
  • Heparin Lyase