The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium

Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9509-14. doi: 10.1073/pnas.132181499. Epub 2002 Jul 1.


The complete genome of the green-sulfur eubacterium Chlorobium tepidum TLS was determined to be a single circular chromosome of 2,154,946 bp. This represents the first genome sequence from the phylum Chlorobia, whose members perform anoxygenic photosynthesis by the reductive tricarboxylic acid cycle. Genome comparisons have identified genes in C. tepidum that are highly conserved among photosynthetic species. Many of these have no assigned function and may play novel roles in photosynthesis or photobiology. Phylogenomic analysis reveals likely duplications of genes involved in biosynthetic pathways for photosynthesis and the metabolism of sulfur and nitrogen as well as strong similarities between metabolic processes in C. tepidum and many Archaeal species.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carbon Dioxide / metabolism
  • Chlorobi / genetics*
  • Chlorobi / metabolism*
  • Chromosomes, Bacterial / genetics
  • Citric Acid Cycle
  • DNA Repair
  • Electron Transport
  • Gene Duplication
  • Genome, Bacterial*
  • Models, Biological
  • Molecular Sequence Data
  • Nitrogen / metabolism
  • Oxidative Stress
  • Photosynthesis
  • Phylogeny
  • Protein Biosynthesis
  • Pyrroles / metabolism
  • Sulfur / metabolism
  • Terpenes / metabolism
  • Tetrapyrroles
  • Transcription, Genetic


  • Pyrroles
  • Terpenes
  • Tetrapyrroles
  • Carbon Dioxide
  • Sulfur
  • Nitrogen

Associated data

  • GENBANK/AE006470