Regulation on maturation and function of dendritic cells by Ganoderma lucidum polysaccharides

Immunol Lett. 2002 Oct 1;83(3):163-9. doi: 10.1016/s0165-2478(02)00087-1.


Ganoderma lucidum polysaccharides (Gl-PS) exhibits a variety of immunomodulatory activities, and dendritic cells (DC) are professional antigen presenting cells, which are pivotal for initiation of primary immune response. In this study, the regulatory effects of Gl-PS on maturation and function of cultured murine bone marrow derived DC were investigated in vitro. Gl-PS (0.8, 3.2, or 12.8 microg/ml) could increase the co-expression of CD11c and I-A/I-E molecules on DC surface, promote mRNA expression of cytokine IL-12 p40 in DC and augment protein production of IL-12 P40 in culture supernatants. The lymphocyte proliferation of mixed lymphocyte culture (MLC) induced by mature DC was enhanced by Gl-PS, either. Gl-PS was shown to promote not only the maturation of cultured murine bone marrow derived DC in vitro, but also the immune response initiation induced by DC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow / drug effects
  • Bone Marrow / immunology
  • Bone Marrow / metabolism
  • CD11c Antigen / biosynthesis
  • Cell Differentiation / drug effects*
  • Cells, Cultured
  • Cytokines / pharmacology
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism
  • Female
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Histocompatibility Antigens Class II / biosynthesis
  • Interleukin-12 / genetics
  • Interleukin-12 Subunit p40
  • Lymphocyte Culture Test, Mixed
  • Male
  • Mice
  • Polysaccharides / pharmacology*
  • Protein Subunits / genetics
  • RNA, Messenger / analysis
  • Reishi
  • Reverse Transcriptase Polymerase Chain Reaction


  • CD11c Antigen
  • Cytokines
  • Histocompatibility Antigens Class II
  • I-E-antigen
  • Interleukin-12 Subunit p40
  • Polysaccharides
  • Protein Subunits
  • RNA, Messenger
  • Interleukin-12