Abstract
Ganoderma lucidum polysaccharides (Gl-PS) exhibits a variety of immunomodulatory activities, and dendritic cells (DC) are professional antigen presenting cells, which are pivotal for initiation of primary immune response. In this study, the regulatory effects of Gl-PS on maturation and function of cultured murine bone marrow derived DC were investigated in vitro. Gl-PS (0.8, 3.2, or 12.8 microg/ml) could increase the co-expression of CD11c and I-A/I-E molecules on DC surface, promote mRNA expression of cytokine IL-12 p40 in DC and augment protein production of IL-12 P40 in culture supernatants. The lymphocyte proliferation of mixed lymphocyte culture (MLC) induced by mature DC was enhanced by Gl-PS, either. Gl-PS was shown to promote not only the maturation of cultured murine bone marrow derived DC in vitro, but also the immune response initiation induced by DC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow / drug effects
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Bone Marrow / immunology
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Bone Marrow / metabolism
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CD11c Antigen / biosynthesis
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Cell Differentiation / drug effects*
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Cells, Cultured
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Cytokines / pharmacology
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Dendritic Cells / cytology
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Dendritic Cells / drug effects*
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Female
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Flow Cytometry
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Fluorescent Antibody Technique
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Histocompatibility Antigens Class II / biosynthesis
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Interleukin-12 / genetics
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Interleukin-12 Subunit p40
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Lymphocyte Culture Test, Mixed
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Male
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Mice
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Polysaccharides / pharmacology*
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Protein Subunits / genetics
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RNA, Messenger / analysis
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Reishi
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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CD11c Antigen
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Cytokines
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Histocompatibility Antigens Class II
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I-E-antigen
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Interleukin-12 Subunit p40
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Polysaccharides
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Protein Subunits
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RNA, Messenger
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Interleukin-12