RNA interference in adult mice

Nature. 2002 Jul 4;418(6893):38-9. doi: 10.1038/418038a.

Abstract

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.

MeSH terms

  • Aging / genetics*
  • Animals
  • Gene Silencing*
  • Genes, Reporter / genetics
  • Hepacivirus / genetics*
  • Humans
  • Liver / metabolism
  • Luciferases / biosynthesis
  • Luciferases / genetics
  • Mice
  • RNA, Double-Stranded / chemistry
  • RNA, Double-Stranded / genetics
  • RNA, Double-Stranded / metabolism
  • RNA, Small Interfering
  • RNA, Untranslated / chemistry
  • RNA, Untranslated / genetics*
  • RNA, Untranslated / metabolism*
  • RNA, Viral / genetics
  • RNA, Viral / metabolism
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Substrate Specificity
  • Transgenes / genetics
  • Viral Nonstructural Proteins / biosynthesis
  • Viral Nonstructural Proteins / genetics

Substances

  • NS-5 protein, hepatitis C virus
  • RNA, Double-Stranded
  • RNA, Small Interfering
  • RNA, Untranslated
  • RNA, Viral
  • Recombinant Fusion Proteins
  • Viral Nonstructural Proteins
  • Luciferases