Ultrastructural study on mitosis and cytokinesis in Scytosiphon lomentaria zygotes (Scytosiphonales, Phaeophyceae) by freeze-substitution

Protoplasma. 2002 May;219(3-4):140-9. doi: 10.1007/s007090200015.

Abstract

The ultrastructure of mitosis and cytokinesis in Scytosiphon lomentaria (Lyngbye) Link zygotes was studied by freeze fixation and substitution. During mitosis, the nuclear envelope remained mostly intact. Spindle microtubules (MTs) from the centrosome passed through the gaps of the nuclear envelope and entered the nucleoplasm. In anaphase and telophase, two daughter chromosome masses were partially surrounded with endoplasmic reticulum. After telophase, the nuclear envelope was reconstructed and two daughter nuclei formed. Then, several large vacuoles occupied the space between the daughter nuclei. MTs from the centrosomes extended toward the mid-plane between two daughter nuclei, among the vacuoles. At that time, Golgi bodies near the centrosome actively produced many vesicles. Midway between the daughter nuclei, small globular vesicles and tubular cisternae accumulated. These vesicles derived from Golgi bodies were transported from the centrosome to the future division plane. Cytokinesis then proceeded by fusion of these vesicles, but not by a furrowing of the plasma membrane. After completion of the continuity with the plasma membrane, cell wall material was deposited between the plasma membranes. The tubular cisternae were still observed at the periphery of the newly formed septum. Microfilaments could not be observed by this procedure. We conclude that cytokinesis in the brown algae proceeds by fusion of Golgi vesicles and tubular cisternae, not by a furrowing of the plasma membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase / physiology
  • Animals
  • Cell Division / genetics
  • Cell Division / physiology
  • Cell Nucleus / genetics
  • Centromere / genetics
  • Female
  • Freeze Substitution / methods
  • Golgi Apparatus / genetics
  • Male
  • Metaphase / physiology
  • Mitosis / genetics
  • Mitosis / physiology
  • Models, Biological
  • Phaeophyta / cytology
  • Phaeophyta / genetics
  • Phaeophyta / ultrastructure*
  • Telophase / physiology
  • Zygote / ultrastructure*