Characterization of granulocyte colony-stimulating factor receptor expressed on human lymphocytes

Br J Haematol. 2002 Jul;118(1):296-304. doi: 10.1046/j.1365-2141.2002.03574.x.


We have studied the characterization of granulocyte colony-stimulating factor receptor (G-CSFR) in human lymphocytes. About one-third to one-quarter of the B lymphocytes from peripheral B-cell sources displayed G-CSF binding on the two-colour immunofluorescence study. The rate of G-CSFR-expressing (G-CSFR+) B cells was higher in bone marrow and cord blood than in peripheral blood, spleen and tonsil. G-CSFR expression was greater in the surface immunoglobulin D (IgD)-positive (sIgD+) B-cell population, but scarce in the sIgD- B-cell population. In tonsil, G-CSFR+ B cells were present among the cells with naive B and germinal-centre B phenotypes, but those with memory B phenotype were rarely found on triple-colour immunofluorescence analysis. Mitogen-activated, but not resting, T lymphocytes also showed G-CSF binding. Several continuous T- and B-cell lines expressed functional G-CSFR, because the addition of G-CSF enhanced the proliferative response of these cell lines. A sequence analysis of G-CSFR mRNA isoforms obtained from the T and B cells revealed that G-CSFR was derived from class I and class IV mRNA. Our results indicated that G-CSFR was constitutively expressed on the B-cell surface and was inducible in T cells.

MeSH terms

  • B-Lymphocytes / chemistry*
  • Bone Marrow Cells
  • Cell Line
  • Child
  • Fetal Blood
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Humans
  • Lymphocyte Activation
  • Palatine Tonsil
  • Protein Binding
  • RNA, Messenger / analysis
  • Receptors, Granulocyte Colony-Stimulating Factor / analysis*
  • Receptors, Granulocyte Colony-Stimulating Factor / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spleen
  • T-Lymphocytes / chemistry*


  • RNA, Messenger
  • Receptors, Granulocyte Colony-Stimulating Factor
  • Granulocyte Colony-Stimulating Factor