A Novel Von Willebrand Factor Binding Protein Expressed by Staphylococcus Aureus

Microbiology. 2002 Jul;148(Pt 7):2037-2044. doi: 10.1099/00221287-148-7-2037.

Abstract

When a shotgun phage-display library of Staphylococcus aureus Newman was affinity selected (panned) against recombinant von Willebrand factor (vWf), a novel von Willebrand factor binding protein (vWbp) was found. Experimental data indicate that the interaction between vWbp and vWf is very specific and mediated by a region of 26 aa residues in the C-terminal part of vWbp. vWbp has an N-terminal secretory signal sequence but no cell wall anchoring motif, suggesting a soluble extracellular location. Mature vWbp could be purified from the culture supernatant and the identity of the protein was confirmed by N-terminal sequencing. vWbp migrates with an apparent molecular mass of 66 kDa and the deduced protein consists of 482 aa. The gene encoding vWbp, named vwb, was present in all S. aureus strains investigated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / metabolism
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Chickens
  • Culture Media, Conditioned / chemistry
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Proteins / metabolism
  • Sequence Analysis, DNA
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / metabolism*
  • Staphylococcus aureus / pathogenicity
  • von Willebrand Factor / genetics
  • von Willebrand Factor / metabolism*

Substances

  • Antibodies
  • Bacterial Proteins
  • Carrier Proteins
  • Culture Media, Conditioned
  • Proteins
  • von Willebrand Factor

Associated data

  • GENBANK/AY032850