Fluorescence in situ hybridization in paraffin tissue sections: pretreatment protocol

Mol Biotechnol. 2002 Jul;21(3):217-20. doi: 10.1385/MB:21:3:217.


Fluorescence in situ hybridization has found wide application in the enumeration of gene and chromosome copy number both in isolated cells and in tissue sections. However, the technique has been less widely used than would be expected in formalin-fixed paraffin processed (archival) tissue. This article describes a method for assessing archival tissue sections, following pretreatment, before applying DNA probes, that gives consistent, reliable results.

Publication types

  • Evaluation Study

MeSH terms

  • Carcinoma / genetics
  • Carcinoma / pathology*
  • Cell Nucleus / ultrastructure
  • Chromosomes, Human / ultrastructure
  • DNA, Neoplasm / analysis
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Paraffin Embedding* / methods
  • Tissue Fixation / methods
  • Urinary Bladder Neoplasms / genetics
  • Urinary Bladder Neoplasms / pathology*


  • DNA, Neoplasm