The identification and subcellular localization of human MRK

Biomol Eng. 2002 Jun;19(1):1-4. doi: 10.1016/s1389-0344(02)00002-3.

Abstract

A novel human kinase gene, human MRK (hMRK), was cloned by using degenerate RT-PCR. The hMRK encoded a putative 632 amino acids protein and was highly homologous to rat MRK (rMRK) in the entire coding region. The hMRK was located at chromosome 6p12.3 by RH-PCR analysis. The hMRK was generally expressed a single approximately 6.3 kb transcript at a low level in a variety of tissues except at a high level in testis. The full-length hMRK protein was fused to C-terminal of GFP and expressed in Hela cells. The fluorescence microscopy results identified its nuclear localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human
  • Chromosomes, Human, Pair 6 / genetics
  • Cloning, Molecular
  • Gene Expression*
  • Humans
  • Molecular Sequence Data
  • Protein Engineering / methods*
  • Protein-Serine-Threonine Kinases / chemical synthesis*
  • Protein-Serine-Threonine Kinases / genetics*
  • Rats
  • Recombinant Fusion Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, Protein / methods*
  • Sequence Homology
  • Tissue Distribution

Substances

  • Recombinant Fusion Proteins
  • Cilk1 protein, rat
  • ICK protein, human
  • Protein-Serine-Threonine Kinases