NF-kappaB activation by hepatitis B virus X (HBx) protein shifts the cellular fate toward survival

Cancer Lett. 2002 Oct 8;184(1):97-104. doi: 10.1016/s0304-3835(02)00187-8.

Abstract

In this paper, we examined the cellular effect of hepatitits B virus X (HBx) in ChangX-34 cells, inducible HBx-expressing cells. High expression of HBx protein in ChangX-34 cells resulted in approximately three-fold increase in DNA synthesis and did not show apoptotic changes. Expression of HBx in these cells was accompanied by the NF-kappaB-mediated transcription. Interestingly, inhibition of NF-kappaB activity either by treatment with sulfasalazine, a specific inhibitor of NF-kappaB, or by expressing IkappaBalpha super-repressor significantly increased cell death in ChangX-34 cells but had no influence on parental Chang cells. Thus, the activation of NF-kappaB in HBx-expressing cells may play a critical role in shifting the balance toward cell survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Anti-Infective Agents / pharmacology
  • Blotting, Western
  • Cell Division / physiology*
  • Cell Survival / physiology*
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Colony-Forming Units Assay
  • Gene Expression Regulation
  • Hepatitis B Antigens / metabolism
  • Hepatitis B Antigens / pharmacology*
  • Humans
  • I-kappa B Proteins / pharmacology
  • Liver / physiology
  • NF-kappa B / metabolism*
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins c-rel
  • RNA, Messenger / metabolism
  • Sulfasalazine / pharmacology
  • Tetracyclines
  • Thymidine / metabolism
  • Trans-Activators / metabolism
  • Trans-Activators / pharmacology*
  • Transcription Factor AP-1
  • Transcription, Genetic
  • Transcriptional Activation

Substances

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Hepatitis B Antigens
  • I-kappa B Proteins
  • NF-kappa B
  • Proto-Oncogene Proteins c-rel
  • RNA, Messenger
  • Tetracyclines
  • Trans-Activators
  • Transcription Factor AP-1
  • hepatitis B virus X protein
  • Sulfasalazine
  • Chloramphenicol O-Acetyltransferase
  • Thymidine