Chromosome 2p, 3p, 5q and 18q status in sporadic gastric cancer

Pathology. 2002 Jun;34(3):275-81. doi: 10.1080/00313020220131354.

Abstract

Aim: The genetic make-up of gastric cancers in low-risk population groups from South Africa is largely unknown. The purpose of this study was to ascertain the incidence of microsatellite instability and loss of heterozygosity in this population.

Methods: Thirty-seven gastrectomy specimens for sporadic gastric cancer were analysed for the following clinicopathological parameters: age, gender, race, histopathological type, size of tumour, lymph node status and the presence/absence of Helicobacter pylori. DNA was then extracted from paraffin-embedded tissue and seven microsatellite markers in 2p, 3p, 5q and 18q loci were examined using automated DNA fluorescent technology.

Results: Only eight cases showed microsatellite instability (MSI) for one marker and were thus categorised as MSI-low. In the 3p region, loss of heterozygosity (LOH) was detected in 21.7-38.3% of informative cases, whilst in the 18q region CLOH ranged from 25 to 38.4%. LOH was not seen in the 2p locus and only one case showed LOH in the 5q region. When the molecular changes were compared with clinicopathological parameters, a statistically significant relationship did not emerge with any single parameter.

Conclusions: This study shows that sporadic gastric cancer from a low-risk population in South Africa is MSI-low or MSI-stable, and that LOH in the 3p and 18q regions is similar to that seen in other low-risk populations from different geographical regions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adult
  • Aged
  • Aged, 80 and over
  • Blacks / genetics
  • Carrier Proteins
  • Chromosomes, Human, 1-3*
  • Chromosomes, Human, Pair 18*
  • Chromosomes, Human, Pair 5*
  • DNA, Neoplasm / analysis
  • DNA-Binding Proteins*
  • Female
  • Genes, APC
  • Genes, DCC
  • Humans
  • India / ethnology
  • Loss of Heterozygosity*
  • Male
  • Microsatellite Repeats
  • Middle Aged
  • MutL Protein Homolog 1
  • MutS Homolog 2 Protein
  • Neoplasm Proteins / genetics
  • Nuclear Proteins
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / genetics
  • South Africa
  • Stomach Neoplasms / ethnology
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / surgery
  • Whites / genetics

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • DNA, Neoplasm
  • DNA-Binding Proteins
  • MLH1 protein, human
  • Neoplasm Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • MSH2 protein, human
  • MutL Protein Homolog 1
  • MutS Homolog 2 Protein