Effect of fur mutation on acid-tolerance response and in vivo virulence of avian septicemic Escherichia coli

Can J Microbiol. 2002 May;48(5):458-62. doi: 10.1139/w02-042.


The Fur (ferric uptake regulator) protein is a master regulator of iron metabolism in gram-negative bacteria. In the present study, the effect of a partial deletion of the fur gene on the acid-tolerance response and in vivo virulence of avian Escherichia coli was examined. The fur mutant was unable to trigger the acid-tolerance response as observed in the wild-type parent strain. However, the mutant was as virulent as the wild-type parent strain when tested in 1-day-old chickens by subcutaneous inoculation. These data indicate that the fur gene is involved in the acid-tolerance response but not involved in the virulence of E. coli, as detected by the ability to cause septicemia in our experimental infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteremia / microbiology
  • Bacteremia / veterinary*
  • Bacterial Proteins / genetics*
  • Chickens
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary
  • Heat-Shock Response*
  • Hydrogen-Ion Concentration
  • Mutation*
  • Poultry Diseases / microbiology*
  • Repressor Proteins / genetics*
  • Virulence


  • Bacterial Proteins
  • Repressor Proteins
  • ferric uptake regulating proteins, bacterial