Abstract
In a genetic screen for altered circadian period length in Arabidopsis, we isolated a mutant with a long free-running period. The tej mutation acts independently of light quality and quantity. It affects clock-controlled transcription of genes in Arabidopsis and alters the timing of the photoperiod-dependent transition from vegetative growth to flowering. Map-based cloning of TEJ identified a poly(ADP-ribose) glycohydrolase (PARG). An inhibitor of poly(ADP-ribosyl)ation rescued the period phenotype of tej mutant and shortened the period length of wild-type plants. Posttranslational poly(ADP-ribosyl)ation of an oscillator component may contribute to setting the period length of the Arabidopsis central oscillator.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Arabidopsis / cytology
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / isolation & purification*
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Arabidopsis Proteins / metabolism
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Benzamides / pharmacology
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Biological Clocks / genetics*
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Circadian Rhythm / genetics*
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Cloning, Molecular
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Enzyme Inhibitors / pharmacology
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Gene Expression Regulation, Plant / drug effects
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Gene Expression Regulation, Plant / physiology
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Glycoside Hydrolases / genetics*
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Glycoside Hydrolases / isolation & purification*
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Glycoside Hydrolases / metabolism
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Molecular Sequence Data
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Mutation / genetics
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Periodicity*
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Phenotype
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Photic Stimulation
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Photoperiod*
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Poly Adenosine Diphosphate Ribose / genetics
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Poly Adenosine Diphosphate Ribose / metabolism*
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
Substances
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Arabidopsis Proteins
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Benzamides
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Enzyme Inhibitors
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Poly Adenosine Diphosphate Ribose
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3-aminobenzamide
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Glycoside Hydrolases
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poly ADP-ribose glycohydrolase