tej defines a role for poly(ADP-ribosyl)ation in establishing period length of the arabidopsis circadian oscillator

Dev Cell. 2002 Jul;3(1):51-61. doi: 10.1016/s1534-5807(02)00200-9.


In a genetic screen for altered circadian period length in Arabidopsis, we isolated a mutant with a long free-running period. The tej mutation acts independently of light quality and quantity. It affects clock-controlled transcription of genes in Arabidopsis and alters the timing of the photoperiod-dependent transition from vegetative growth to flowering. Map-based cloning of TEJ identified a poly(ADP-ribose) glycohydrolase (PARG). An inhibitor of poly(ADP-ribosyl)ation rescued the period phenotype of tej mutant and shortened the period length of wild-type plants. Posttranslational poly(ADP-ribosyl)ation of an oscillator component may contribute to setting the period length of the Arabidopsis central oscillator.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / isolation & purification*
  • Arabidopsis Proteins / metabolism
  • Benzamides / pharmacology
  • Biological Clocks / genetics*
  • Circadian Rhythm / genetics*
  • Cloning, Molecular
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation, Plant / drug effects
  • Gene Expression Regulation, Plant / physiology
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / isolation & purification*
  • Glycoside Hydrolases / metabolism
  • Molecular Sequence Data
  • Mutation / genetics
  • Periodicity*
  • Phenotype
  • Photic Stimulation
  • Photoperiod*
  • Poly Adenosine Diphosphate Ribose / genetics
  • Poly Adenosine Diphosphate Ribose / metabolism*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid


  • Arabidopsis Proteins
  • Benzamides
  • Enzyme Inhibitors
  • Poly Adenosine Diphosphate Ribose
  • 3-aminobenzamide
  • Glycoside Hydrolases
  • poly ADP-ribose glycohydrolase

Associated data

  • GENBANK/AF394690