Transmitter-evoked local calcium release stabilizes developing dendrites

Nature. 2002 Jul 11;418(6894):177-81. doi: 10.1038/nature00850.


In the central nervous system, dendritic arborizations of neurons undergo dynamic structural remodelling during development. Processes are elaborated, maintained or eliminated to attain the adult pattern of synaptic connections. Although neuronal activity influences this remodelling, it is not known how activity exerts its effects. Here we show that neurotransmission-evoked calcium (Ca(2+)) release from intracellular stores stabilizes dendrites during the period of synapse formation. Using a ballistic labelling method to load cells with Ca(2+) indicator dyes, we simultaneously monitored dendritic activity and structure in the intact retina. Two distinct patterns of spontaneous Ca(2+) increases occurred in developing retinal ganglion cells--global increases throughout the arborization, and local 'flashes' of activity restricted to small dendritic segments. Blockade of local, but not global, activity caused rapid retraction of dendrites. This retraction was prevented locally by focal uncaging of caged Ca(2+) that triggered Ca(2+) release from internal stores. Thus, local Ca(2+) release is a mechanism by which afferent activity can selectively and differentially regulate dendritic structure across the developing arborization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylcholine / pharmacology
  • Amacrine Cells / cytology
  • Amacrine Cells / drug effects
  • Amacrine Cells / metabolism
  • Animals
  • Atropine / pharmacology
  • Calcium / metabolism*
  • Calcium Signaling / drug effects*
  • Cell Size / drug effects
  • Chick Embryo
  • Dendrites / drug effects*
  • Dendrites / metabolism*
  • Egtazic Acid / analogs & derivatives
  • Egtazic Acid / pharmacology
  • Excitatory Amino Acid Antagonists / pharmacology
  • Indoles / pharmacology
  • Neurites / drug effects
  • Neurites / metabolism
  • Neurotransmitter Agents / pharmacology*
  • Nicotinic Antagonists / pharmacology
  • Patch-Clamp Techniques
  • Retinal Ganglion Cells / cytology
  • Retinal Ganglion Cells / drug effects
  • Retinal Ganglion Cells / metabolism
  • Ryanodine / pharmacology
  • Synapses / drug effects
  • Synapses / metabolism
  • Tetrodotoxin / pharmacology
  • Thapsigargin / pharmacology


  • Excitatory Amino Acid Antagonists
  • Indoles
  • Neurotransmitter Agents
  • Nicotinic Antagonists
  • Ryanodine
  • 2-nitrophenyl-EGTA
  • Tetrodotoxin
  • Egtazic Acid
  • Thapsigargin
  • Atropine
  • Acetylcholine
  • Calcium
  • cyclopiazonic acid