Molecular characterization of local Bacillus thuringiensis strains recovered from Northern Jordan

J Basic Microbiol. 2002;42(3):156-61. doi: 10.1002/1521-4028(200206)42:3<156::AID-JOBM156>3.0.CO;2-0.

Abstract

A total of 80 isolates of Bacillus thuringiensis were recovered from different habitats of Northern Jordan. These isolates were grouped into three classes based on crystal morphology (spherical, bipyramidal, and both bipyramidal and cuboidal). The isolates that produced spherical crystals were the most common and the most toxic to diptera. SDS-PAGE analysis of the isolates and some reference strains with similar crystal morphology showed similar protein profiles with heterogeneous multiple protein components. The plasmid DNA content of the isolates in comparison with B. thuringiensis serovar israelensis gave a similar single intense DNA band.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacillus thuringiensis / classification*
  • Bacillus thuringiensis / genetics*
  • Bacillus thuringiensis / growth & development
  • Bacillus thuringiensis / isolation & purification
  • Bacillus thuringiensis Toxins
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / pharmacology
  • Bacterial Toxins*
  • Biological Assay
  • Culex / drug effects
  • Drosophila melanogaster / drug effects
  • Endotoxins / chemistry
  • Endotoxins / pharmacology
  • Environmental Microbiology*
  • Hemolysin Proteins
  • Inclusion Bodies / chemistry
  • Jordan
  • Plasmids / genetics

Substances

  • Bacillus thuringiensis Toxins
  • Bacterial Proteins
  • Bacterial Toxins
  • Endotoxins
  • Hemolysin Proteins
  • insecticidal crystal protein, Bacillus Thuringiensis