Decreased catecholamine-induced cell death in B lymphocytes from patients with rheumatoid arthritis

Ann N Y Acad Sci. 2002 Jun;966:425-8. doi: 10.1111/j.1749-6632.2002.tb04243.x.

Abstract

The expression of beta2-adrenergic receptors (beta2-R) on B lymphocytes and agonist-induced cAMP production is reduced in patients with rheumatoid arthritis (RA). To further study functional consequences of the diminished beta2-R density on B lymphocytes in RA patients, agonist-induced cell death was evaluated and compared to healthy controls. B lymphocytes from patients with RA and healthy controls were activated with anti-IgM-antibody. Coincubation was carried out with isoprenaline (iso, 0.001-10 microM). Apoptotic and necrotic cells were determined using Annexin-V and propidium-iodide staining. beta2-R-induced cell death in B cells from healthy volunteers was stimulated after 24 h (medium, 21.2 +/- 1.6%; iso, 34.6 +/- 4.4%; increase 59.3 +/- 10.1%). However, in RA patients the increase in cell death following beta2-R stimulation (21.8 +/- 8.9%) was significantly impaired (p = 0.02). Our data demonstrate that catecholamine-induced cell death after stimulation of beta2-R on B lymphocytes is decreased in RA patients, possibly contributing to the pathogenesis of the disease.

MeSH terms

  • Adrenergic beta-Agonists / pharmacology*
  • Annexin A5 / analysis
  • Antibodies, Anti-Idiotypic / pharmacology
  • Apoptosis / drug effects*
  • Arthritis, Rheumatoid / immunology
  • Arthritis, Rheumatoid / pathology*
  • Autoimmune Diseases / immunology
  • Autoimmune Diseases / pathology*
  • B-Lymphocytes / pathology*
  • Biomarkers
  • Caspase 3
  • Caspases / analysis
  • Cells, Cultured / drug effects
  • Drug Resistance
  • Epinephrine / pharmacology
  • Humans
  • Isoproterenol / pharmacology
  • Receptors, Adrenergic, beta-2 / drug effects
  • Terbutaline / pharmacology

Substances

  • Adrenergic beta-Agonists
  • Annexin A5
  • Antibodies, Anti-Idiotypic
  • Biomarkers
  • Receptors, Adrenergic, beta-2
  • anti-IgM
  • CASP3 protein, human
  • Caspase 3
  • Caspases
  • Isoproterenol
  • Terbutaline
  • Epinephrine