A nucleic acid sequence-based amplification method to detect Salmonella enterica serotype enteritidis strain PT4 in liquid whole egg

N Cook; J Ellison; A S Kurdziel; S Simpkins; J P Hays

doi:10.4315/0362-028x-65.7.1177

A nucleic acid sequence-based amplification method to detect Salmonella enterica serotype enteritidis strain PT4 in liquid whole egg

J Food Prot. 2002 Jul;65(7):1177-8. doi: 10.4315/0362-028x-65.7.1177.

Authors

N Cook¹, J Ellison, A S Kurdziel, S Simpkins, J P Hays

Affiliation

¹ Central Science Laboratory, Sand Hutton, York, UK. n.cook@csl.gov.uk

PMID: 12117254
DOI: 10.4315/0362-028x-65.7.1177

Abstract

Nucleic acid sequence-based amplification (NASBA) was applied to the detection of Salmonella enterica cells in liquid whole egg. Samples (25 g) of liquid whole egg inoculated with Salmonella cells were enriched for 16 h in buffered peptone water, and the NASBA procedure was effective in detecting the presence of Salmonella in samples inoculated with 10 to 100 CFU prior to enrichment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Colony Count, Microbial
Eggs / microbiology*
Food Microbiology
Nucleic Acid Amplification Techniques
RNA, Bacterial / chemistry*
RNA, Bacterial / isolation & purification
Salmonella enteritidis / genetics*
Self-Sustained Sequence Replication / methods*
Sequence Analysis, RNA

Substances

RNA, Bacterial