A review of aerobic and psychrotrophic plate count procedures for fresh meat and poultry products

J Food Prot. 2002 Jul;65(7):1200-6. doi: 10.4315/0362-028x-65.7.1200.

Abstract

This is a review of reports that employed aerobic plate counts on fresh meat and poultry products since 1985; it lists synopses of 100 applications. A total of 15 different plating media were used, with 48 (48%) being either plate count agar (PCA) or tryptone glucose yeast extract agar. The temperature-time relations ranged from a low temperature of 20 degrees C for 120 h to 37 degrees C for 24 h. Some 29 different temperature-time combinations were used among the total of 109, with 21 (19.3%) being 35 degrees C/48 h, followed by 12 (11.0%) at 32 degrees C/48 h, 11 (10.1%) at 25 degrees C/48 h, and 9 (8.3%) at 25 degrees C/72 h. Fifty-four (49.5%) plate count applications employed incubation temperatures of 30 degrees C and below. From the 26 reports that employed psychrotrophic counts, 16 (61.5%) used PCA; 18 different temperature-time combinations were used, with 7 degrees C/10 d employed by only four. Twenty-one (80.8%) employed an incubation temperature at or <10 degrees C, and five employed an incubation temperature >10 degrees C. There is a serious need for some consensus on methodologies for aerobic and psychrotrophic counts on fresh meat and poultry products.

Publication types

  • Review

MeSH terms

  • Agar / chemistry
  • Animals
  • Bacteria, Aerobic / growth & development*
  • Bacteria, Aerobic / isolation & purification
  • Colony Count, Microbial
  • Culture Media / chemistry
  • Food Microbiology
  • Meat Products / microbiology*
  • Poultry Products / microbiology*
  • Temperature
  • Time Factors

Substances

  • Culture Media
  • Agar