Immobilization of invertase on rice husk using polyethylenimine

J Biochem Biophys Methods. 2002 Jun 28;52(1):59-62. doi: 10.1016/s0165-022x(02)00032-5.

Abstract

Washed and dried rice husk was coated with 2% polyethylenimine (PEI). Invertase was immobilized on this support through adsorption followed by cross-linking with 2% glutaraldehyde. Immobilized enzyme was reused for the hydrolysis of sucrose without loss in activity. This approach may serve as a simple technique in the future for the covalent immobilization of enzymes on lignocellulosic supports.

MeSH terms

  • Adsorption
  • Enzymes, Immobilized
  • Glutaral / chemistry
  • Glycoside Hydrolases / chemistry*
  • Hydrolysis
  • Oryza / enzymology*
  • Polyethyleneimine / chemistry*
  • Sucrose / chemistry
  • beta-Fructofuranosidase

Substances

  • Enzymes, Immobilized
  • Sucrose
  • Polyethyleneimine
  • Glycoside Hydrolases
  • beta-Fructofuranosidase
  • Glutaral