Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

doi:10.1128/AAC.46.8.2720-2722.2002

. 2002 Aug;46(8):2720-2.

doi: 10.1128/AAC.46.8.2720-2722.2002.

Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

Juan-Carlos Palomino¹, Anandi Martin, Mirtha Camacho, Humberto Guerra, Jean Swings, Françoise Portaels

Affiliations

PMID: 12121966
PMCID: PMC127336
DOI: 10.1128/AAC.46.8.2720-2722.2002

Free PMC article

Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

Juan-Carlos Palomino et al. Antimicrob Agents Chemother. 2002 Aug.

Free PMC article

. 2002 Aug;46(8):2720-2.

doi: 10.1128/AAC.46.8.2720-2722.2002.

Authors

Juan-Carlos Palomino¹, Anandi Martin, Mirtha Camacho, Humberto Guerra, Jean Swings, Françoise Portaels

Affiliation

¹ Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium. palomino@itg.be

PMID: 12121966
PMCID: PMC127336
DOI: 10.1128/AAC.46.8.2720-2722.2002

Abstract

A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

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References

1. Canetti, G., S. Froman, J. Grosset, P. Hauduroy, M. Langerova, H. T. Mahler, G. Meissner, D. A. Mitchison, and L. Sula. 1963. Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull. W. H. O. 29:565-578. - PMC - PubMed
1. Canetti, G., W. Fox, A. Khomenko, H. T. Mahler, N. K. Menon, D. A. Mitchison, N. Rist, and N. A. Smelev. 1969. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programs. Bull. W. H. O. 41:21-43. - PMC - PubMed
1. Carter, R. A., S. A. Ericsson, C. D. Corn, P. R. Weyerts, M. G. Dart, S. G. Escue, and J. Mesta. 1998. Assessing the fertility potential of equine semen samples using the reducible dyes methylene green and resazurin. Arch. Androl. 40:59-66. - PubMed
1. Coates, A. R., and D. A. Mitchison. 1983. The role of sensitivity tests in short-term chemotherapy. Bull. Int. Union Tuberc. 58:111-114. - PubMed
1. Collins, L., and S. G. Franzblau. 1997. Microplate Alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob. Agents Chemother. 41:1004-1009. - PMC - PubMed

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[1] Canetti, G., S. Froman, J. Grosset, P. Hauduroy, M. Langerova, H. T. Mahler, G. Meissner, D. A. Mitchison, and L. Sula. 1963. Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull. W. H. O. 29:565-578. - PMC - PubMed

[2] Canetti, G., S. Froman, J. Grosset, P. Hauduroy, M. Langerova, H. T. Mahler, G. Meissner, D. A. Mitchison, and L. Sula. 1963. Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull. W. H. O. 29:565-578. - PMC - PubMed

[3] Canetti, G., W. Fox, A. Khomenko, H. T. Mahler, N. K. Menon, D. A. Mitchison, N. Rist, and N. A. Smelev. 1969. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programs. Bull. W. H. O. 41:21-43. - PMC - PubMed

[4] Canetti, G., W. Fox, A. Khomenko, H. T. Mahler, N. K. Menon, D. A. Mitchison, N. Rist, and N. A. Smelev. 1969. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programs. Bull. W. H. O. 41:21-43. - PMC - PubMed

[5] Carter, R. A., S. A. Ericsson, C. D. Corn, P. R. Weyerts, M. G. Dart, S. G. Escue, and J. Mesta. 1998. Assessing the fertility potential of equine semen samples using the reducible dyes methylene green and resazurin. Arch. Androl. 40:59-66. - PubMed

[6] Carter, R. A., S. A. Ericsson, C. D. Corn, P. R. Weyerts, M. G. Dart, S. G. Escue, and J. Mesta. 1998. Assessing the fertility potential of equine semen samples using the reducible dyes methylene green and resazurin. Arch. Androl. 40:59-66. - PubMed

[7] Coates, A. R., and D. A. Mitchison. 1983. The role of sensitivity tests in short-term chemotherapy. Bull. Int. Union Tuberc. 58:111-114. - PubMed

[8] Coates, A. R., and D. A. Mitchison. 1983. The role of sensitivity tests in short-term chemotherapy. Bull. Int. Union Tuberc. 58:111-114. - PubMed

[9] Collins, L., and S. G. Franzblau. 1997. Microplate Alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob. Agents Chemother. 41:1004-1009. - PMC - PubMed

[10] Collins, L., and S. G. Franzblau. 1997. Microplate Alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob. Agents Chemother. 41:1004-1009. - PMC - PubMed

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Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

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Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

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