Abstract
The mammalian homologue of the Drosophila axonal guidance receptor roundabout is expressed in a wide range of tissues. Here we show that alternative splicing of the Dutt1/Robo1 gene results in two mRNA transcripts with different signal peptides, which are differentially expressed throughout mouse embryogenesis. Since mice with a targeted deletion in the Dutt1/Robo1 gene have abnormal lung pathology, immunohistochemistry was used to identify the cellular expression pattern of Dutt1/Robo1 during lung development. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing*
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Amino Acid Sequence
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Animals
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Bronchi / growth & development
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Bronchi / metabolism*
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Epithelium / growth & development
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Epithelium / metabolism
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Gene Deletion
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Gene Expression Regulation, Developmental*
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Immunohistochemistry
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Lung / embryology
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Lung / growth & development
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Lung / metabolism*
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins
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Protein Isoforms / biosynthesis
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Protein Isoforms / genetics
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Protein Sorting Signals
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RNA, Messenger / chemistry
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RNA, Messenger / metabolism
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Receptors, Immunologic / biosynthesis
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Receptors, Immunologic / genetics*
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Roundabout Proteins
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Tissue Distribution
Substances
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Nerve Tissue Proteins
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Protein Isoforms
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Protein Sorting Signals
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RNA, Messenger
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Receptors, Immunologic
Associated data
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GENBANK/AF040990
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GENBANK/Z95705