Temporal and spatial expression of two isoforms of the Dutt1/Robo1 gene in mouse development

FEBS Lett. 2002 Jul 17;523(1-3):12-6. doi: 10.1016/s0014-5793(02)02904-6.

Abstract

The mammalian homologue of the Drosophila axonal guidance receptor roundabout is expressed in a wide range of tissues. Here we show that alternative splicing of the Dutt1/Robo1 gene results in two mRNA transcripts with different signal peptides, which are differentially expressed throughout mouse embryogenesis. Since mice with a targeted deletion in the Dutt1/Robo1 gene have abnormal lung pathology, immunohistochemistry was used to identify the cellular expression pattern of Dutt1/Robo1 during lung development. Dutt1/Robo1 expression was widespread and diffuse in the lung at embryonic day 17.5 but became increasingly localised to the bronchial epithelium in newborn and adult mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Bronchi / growth & development
  • Bronchi / metabolism*
  • Epithelium / growth & development
  • Epithelium / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Developmental*
  • Immunohistochemistry
  • Lung / embryology
  • Lung / growth & development
  • Lung / metabolism*
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Protein Isoforms / biosynthesis
  • Protein Isoforms / genetics
  • Protein Sorting Signals
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • Receptors, Immunologic / biosynthesis
  • Receptors, Immunologic / genetics*
  • Tissue Distribution

Substances

  • Nerve Tissue Proteins
  • Protein Isoforms
  • Protein Sorting Signals
  • RNA, Messenger
  • Receptors, Immunologic
  • roundabout protein

Associated data

  • GENBANK/AF040990
  • GENBANK/Z95705