Generation of a permanent cell line that supports efficient growth of Marek's disease virus (MDV) by constitutive expression of MDV glycoprotein E

J Gen Virol. 2002 Aug;83(Pt 8):1987-1992. doi: 10.1099/0022-1317-83-8-1987.


A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marek's disease virus serotype 1 (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CVI988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marek's disease. Chickens were immunized at 1 day old with 10(3) p.f.u. CVI988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marek's disease, only 1/8 animals from both CVI988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Mardivirus / growth & development*
  • Mardivirus / immunology
  • Mardivirus / pathogenicity
  • Marek Disease / prevention & control*
  • Marek Disease / virology
  • Muscles / cytology
  • Poultry Diseases / prevention & control
  • Poultry Diseases / virology
  • Quail
  • Tumor Cells, Cultured
  • Vaccination
  • Viral Envelope Proteins / biosynthesis*
  • Viral Vaccines*
  • Virology / methods
  • Virulence
  • Virus Cultivation*
  • Virus Replication


  • Viral Envelope Proteins
  • Viral Vaccines
  • glycoprotein E, Marek's disease virus