A colon tumor model for anticancer agent evaluation

Cancer. 1975 Dec;36(6 Suppl):2441-4. doi: 10.1002/1097-0142(197512)36:6<2441::aid-cncr2820360627>3.0.co;2-p.


Colon tumors of mice were induced with 1, 2-dimethylhydrazine, N-nitroso-N-methylurethane, methylnitrosourea, and 4-methyl-N'-nitro-N-nitrosoguanidine. Of 82 transplantation attempts, four were successful, and the four tumors have been maintained as distinct tumor lines by subcutaneous transplantation. The tumors graded from II, adenocarcinoma, to IV, undifferentiated carcinoma. Volume-doubling times varied over a three-fold range, and metastatic potential ranged from 5 to about 100%. The tumors responded to treatment with some of the same agents found to be active in man. These included cyclophosphamide, 5-fluorouracil, and certain nitrosoureas. Preliminary evidence suggests that these tumor lines may be useful as experimental models.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antineoplastic Agents / therapeutic use*
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / drug therapy*
  • Dimethylhydrazines
  • Disease Models, Animal*
  • Methylnitronitrosoguanidine
  • Methylnitrosourea
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Neoplasm Transplantation
  • Neoplasms, Experimental
  • Nitrosomethylurethane
  • Transplantation, Homologous


  • Antineoplastic Agents
  • Dimethylhydrazines
  • Methylnitronitrosoguanidine
  • Nitrosomethylurethane
  • Methylnitrosourea