Characterization of a renal cell carcinoma cell line derived from a human bone metastasis and establishment of an experimental nude mouse model

J Urol. 2002 Aug;168(2):774-9.


Purpose: We provide the necessary tools to study the biology of bone metastasis using renal cell carcinoma as a model. A relevant renal cell carcinoma cell line developed from a human bone metastasis is described and an experimental model in the nude mouse was established.

Materials and methods: The novel cell line RBM1 was developed from bone metastasis from a patient with renal cell carcinoma. In vitro methods used to study the cell line included karyotype analysis, reverse-transcriptase polymerase chain reaction, ribonuclease protection assay and Western blot analysis. An intratibial injection model in the nude mouse resulted in bone lesions similar to those in human patients.

Results: Chromosomal analysis of this cell line revealed characteristic cytogenetic abnormalities common in renal cell carcinoma. RBM1 cells expressed parathyroid hormone parathyroid hormone related peptide, interleukin-6 and macrophage colony-stimulating factor, which are cytokines involved in osteoclast activation and subsequent bone resorption. Western blot analysis revealed the presence of high levels of epidermal growth factor receptor and c-MET. Reproducible osteolytic lesions developed in the nude mouse after injecting 1 x 106 cells into the tibia.

Conclusions: This cell line and animal model may allow further study of the biology and mechanism of renal cell carcinoma bone metastasis.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / genetics
  • Bone Neoplasms / genetics
  • Bone Neoplasms / pathology
  • Bone Neoplasms / secondary
  • Bone Resorption / genetics
  • Bone Resorption / pathology
  • Carcinoma, Renal Cell / genetics
  • Carcinoma, Renal Cell / pathology
  • Carcinoma, Renal Cell / secondary*
  • Cytokines / analysis
  • Cytokines / genetics
  • Disease Models, Animal
  • ErbB Receptors / analysis
  • ErbB Receptors / genetics
  • Gene Expression Regulation, Neoplastic / physiology
  • Humans
  • Karyotyping
  • Kidney Neoplasms / genetics
  • Kidney Neoplasms / pathology*
  • Male
  • Mice
  • Mice, Nude
  • Middle Aged
  • Neoplasm Transplantation
  • Proto-Oncogene Proteins c-met / analysis
  • Proto-Oncogene Proteins c-met / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tibia / pathology
  • Tumor Cells, Cultured / pathology*


  • Biomarkers, Tumor
  • Cytokines
  • ErbB Receptors
  • Proto-Oncogene Proteins c-met