Use of an oil-hydraulic microinjection pump for subretinal infusions

Ophthalmic Surg Lasers. 2002 Jul-Aug;33(4):340-2.

Abstract

The injection of cell suspensions or drugs into the subretinal space is a new promising option of vitreoretinal surgery for the treatment of degenerative retinal disorders. We used a manual oil-hydraulic microinjection pump to subretinally inject suspensions of retinal pigment epithelial cells in Royal College of Surgeons rats and in patients suffering from age-related macular degeneration with geographic atrophy. The histological examination of the treated rat eyes showed that cell suspensions could be placed precisely in the subretinal space. Intra- and postoperative outcome of the patients in the clinical trial revealed no retinal complications during 6 months of follow up. We suggest the oil-hydraulic microinjection pump to be a valuable instrument for controlled and precisely dosed atraumatic infusion or aspiration of small volumes of cell suspensions, fluids or drugs in vitreoretinal surgery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Transplantation / instrumentation*
  • Cell Transplantation / methods*
  • Cells, Cultured
  • Equipment Design
  • Feasibility Studies
  • Humans
  • Infusion Pumps*
  • Macular Degeneration / surgery*
  • Microinjections / instrumentation*
  • Pigment Epithelium of Eye / cytology*
  • Rats
  • Rats, Inbred Strains
  • Retina / surgery*
  • Safety