Correlation between laminin-5 gamma2 chain expression and epidermal growth factor receptor expression and its clinicopathological significance in squamous cell carcinoma of the tongue

Oncology. 2002;62(4):318-26. doi: 10.1159/000065063.

Abstract

Recent investigations have revealed that growth factors may influence the invasive activity of tumor cells. Expression of laminin-5 gamma2 chain (LN-5 gamma2) and epidermal growth factor receptor (EGFR) in squamous cell carcinomas of the tongue in 104 patients with stage II, III, and IVA, B (excluding the cases with distant metastasis) was examined immunohistochemically to determine the correlation between the two molecules and the associations with the clinicopathological features of each tumor. LN-5 gamma2 expression was clearly demonstrated in the cytoplasm and EGFR in the cell membranes of cancer cells. A significant increase in positivity for LN-5 gamma2 was observed in tumors showing poor differentiation (p < 0.001), infiltrative growth (p < 0.001), and deep invasion (p = 0.038). In a multivariate analysis, increased positivity for LN-5 gamma2 was an independent predictor of an unfavorable outcome (p < 0.001). A significant increase in positivity for EGFR was observed in tumors showing infiltrative growth (p = 0.032) and poor prognosis (p = 0.008). The LN-5 gamma2 expression was correlated significantly with EGFR expression (p < 0.001). Patients with tumor positivity for both molecules showed the worst prognosis (p < 0.001). LN-5 gamma2 overexpression and EGFR overexpression is evident in tumors showing infiltrative growth, suggesting that EGFR may influence the invasive activity of tumor cells through overexpression of LN-5 gamma2.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / surgery
  • ErbB Receptors / metabolism*
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Laminin / metabolism*
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Tongue Neoplasms / metabolism*
  • Tongue Neoplasms / pathology
  • Tongue Neoplasms / surgery

Substances

  • LAMC2 protein, human
  • Laminin
  • ErbB Receptors