New pollen-specific receptor kinases identified in tomato, maize and Arabidopsis: the tomato kinases show overlapping but distinct localization patterns on pollen tubes

Plant Mol Biol. 2002 Sep;50(1):1-16. doi: 10.1023/a:1016077014583.

Abstract

We previously characterized LePRK1 and LePRK2, pollen-specific receptor kinases from tomato (Muschietti et al., 1998). Here we identify a similar receptor kinase from maize, ZmPRK1, that is also specifically expressed late in pollen development, and a third pollen receptor kinase from tomato, LePRK3. LePRK3 is less similar to LePRK1 and LePRK2 than either is to each other. We used immunolocalization to show that all three LePRKs localize to the pollen tube wall, in partially overlapping but distinct patterns. We used RT-PCR and degenerate primers to clone homologues of the tomato kinases from other Solanaceae. We deduced features diagnostic of pollen receptor kinases and used these criteria to identify family members in the Arabidopsis database. RT-PCR confirmed pollen expression for five of these Arabidopsis candidates; two of these are clearly homologues of LePRK3. Our results reveal the existence of a distinct pollen-specific receptor kinase gene family whose members are likely to be involved in perceiving extracellular cues during pollen tube growth.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Arabidopsis / genetics
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Glycosylation
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Lycopersicon esculentum / enzymology
  • Lycopersicon esculentum / genetics
  • Molecular Sequence Data
  • Phylogeny
  • Plant Proteins / genetics
  • Plants / enzymology
  • Plants / genetics*
  • Pollen / enzymology*
  • Pollen / growth & development
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Solanaceae / enzymology
  • Solanaceae / genetics
  • Zea mays / enzymology
  • Zea mays / genetics

Substances

  • DNA, Complementary
  • Isoenzymes
  • Plant Proteins
  • Receptor Protein-Tyrosine Kinases

Associated data

  • GENBANK/AF243040
  • GENBANK/AF243041
  • GENBANK/AF246964
  • GENBANK/AF246965
  • GENBANK/AF246966
  • GENBANK/AF246967
  • GENBANK/AF246968
  • GENBANK/AF246969
  • GENBANK/AF246970
  • GENBANK/AF252412
  • GENBANK/AF252413
  • GENBANK/AF252414
  • GENBANK/AF252415
  • GENBANK/AF267178