Specific IgE determination to Ani s 1, a major allergen from Anisakis simplex, is a useful tool for diagnosis

Ann Allergy Asthma Immunol. 2002 Jul;89(1):74-7. doi: 10.1016/S1081-1206(10)61914-X.


Background: The most serious limitation of the serodiagnosis of parasitoses is the occurrence of cross-reactions.

Objective: The possible use of Anisakis simplex major allergen Ani s 1 for diagnosis.

Patients and methods: Forty-nine non-fish-allergic patients with Anisakis simplex hypersensitivity, 21 patients without allergic episodes suffering intestinal anisakiasis with obstruction of the intestinal lumen, and 10 unrelated sera as a control were included in this study to determine specific immunoglobulin (Ig)E and IgG to Anisakis simplex major allergen Ani s 1 by immunoblotting.

Results: Eighty-six percent of patients with Anisakis simplex hypersensitivity showed specific IgE directed to Ani s 1. Identical result was obtained for IgG detection in this group. Among patients with intestinal anisakiasis, 86% showed specific IgE, but only 29% had specific IgG (P < 0.001, two-tailed Fisher exact test). One of the 10 control subjects was positive both for IgE and IgG (P < 0.001).

Conclusions: Determination of specific IgE directed to Anisakis simplex major allergen Ani s 1 is a useful tool for the diagnosis of hypersensitivity and intestinal anisakiasis. Further, measurement of specific IgG directed to Anisakis simplex major allergen Ani s 1 is only valid for Anisakis simplex allergy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens*
  • Animals
  • Anisakiasis / diagnosis*
  • Anisakis / immunology*
  • Calcium-Binding Proteins / immunology*
  • Fishes / parasitology
  • Helminth Proteins / immunology*
  • Humans
  • Immunoglobulin E / blood*
  • Immunoglobulin G / blood


  • Allergens
  • Calcium-Binding Proteins
  • Helminth Proteins
  • Immunoglobulin G
  • troponin-like protein, Anisakis simplex
  • Immunoglobulin E