Genome stability and the processing of damaged replication forks by RecG

Trends Genet. 2002 Aug;18(8):413-9. doi: 10.1016/s0168-9525(02)02720-8.


Chromosomal duplication faces many blocks to replication fork progression that could destabilize the genome and prove fatal if not overcome. Overcoming such blocks requires interplay between DNA replication, recombination and repair. The RecG protein of Escherichia coli promotes rescue of damaged forks by catalysing their unwinding and conversion to Holliday junctions. Subsequent processing of this structure allows repair or bypass of the fork block, enabling replication to resume without recourse to potentially mutagenic translesion synthesis or recombination. Such direct rescue of stalled forks might help safeguard genome integrity in all organisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • DNA Damage*
  • DNA Helicases*
  • DNA Repair*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Endodeoxyribonucleases / genetics
  • Endodeoxyribonucleases / metabolism
  • Escherichia coli / genetics
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism*
  • Genome, Bacterial
  • Replication Protein A


  • Bacterial Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Replication Protein A
  • RuvB protein, Bacteria
  • ruvC protein, E coli
  • RecG protein, E coli
  • Endodeoxyribonucleases
  • Holliday junction DNA helicase, E coli
  • DNA Helicases