Abstract
Cloned T9 glioma cells (T9-C2) expressing the membrane form of macrophage colony stimulating factor (mM-CSF) inoculated subcutaneously into rats do not grow and glioma-specific immunity is stimulated. Immunotherapy experiments showed that intracranial T9 tumors present for one to four days could be successfully eradicated by peripheral vaccination with T9-C2 cells. CD4+ and CD8+ T splenocytes from immunized rats, when restimulated in vitro with T9 cells, produced interleukin-2 and -4. Protective immunity against intracranial T9 gliomas could only be adoptively transferred into naive rats by the CD4+ splenocytes obtained from T9-C2 immunized rats. Rats immunized by the T9-C2 tumor cells also resisted two different syngeneic gliomas (RT2 and F98) but allowed a syngeneic NUTU-19 ovarian cancer to grow. Such cross-protective immunity against unrelated gliomas suggests that mM-CSF transfected tumor cells have immunotherapeutic potential for use as an allogeneic tumor vaccine.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adoptive Transfer
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Animals
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Brain Neoplasms / immunology*
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Brain Neoplasms / prevention & control
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Brain Neoplasms / ultrastructure
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / transplantation
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Cells, Cultured
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Clone Cells
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Female
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Glioma / immunology*
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Glioma / prevention & control
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Glioma / ultrastructure
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Interleukin-2 / biosynthesis
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Interleukin-2 / genetics
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Interleukin-4 / biosynthesis
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Interleukin-4 / genetics
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Kinetics
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Macrophage Colony-Stimulating Factor / genetics*
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Macrophage Colony-Stimulating Factor / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Neoplasm Transplantation
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RNA, Messenger / biosynthesis
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Rats
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Rats, Inbred F344
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Survival Analysis
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Transfection
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Tumor Cells, Cultured
Substances
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Interleukin-2
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Membrane Proteins
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RNA, Messenger
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Interleukin-4
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Macrophage Colony-Stimulating Factor