Genome-wide transcriptional profiling in a histidine kinase mutant of Helicobacter pylori identifies members of a regulon

J Bacteriol. 2002 Aug;184(16):4630-5. doi: 10.1128/JB.184.16.4630-4635.2002.

Abstract

To identify putative members of a regulon controlled by the H. pylori sensory histidine kinase HP0164 (HK0164), we constructed HK0164 null mutant H. pylori strains and analyzed bacterial gene transcription using DNA arrays. Seven genes were differentially expressed in multiple HK0164 mutant strains compared to their expression in control strains. Strain-dependent variations in differential expression were also detected. These results indicate that the signal transduction circuit utilizing HK0164 controls the transcription of at least seven genes in H. pylori.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Gene Expression Regulation, Bacterial / physiology
  • Genome, Bacterial*
  • Helicobacter pylori / enzymology*
  • Helicobacter pylori / genetics*
  • Histidine Kinase
  • Mutation / physiology
  • Oligonucleotide Array Sequence Analysis
  • Protein Kinases / genetics*
  • Regulon / genetics*
  • Signal Transduction / genetics
  • Transcription, Genetic / genetics

Substances

  • Protein Kinases
  • Histidine Kinase